Keywords

2658-6649

2658-6657

Siberian Journal of Life Sciences and Agriculture

Science and Innovation Center Publishing House

10.12731/2658-6649-2025-17-5-1251

JTFJUD

https://discover-journal.ru/jour/index.php/sjlsa/article/view/1251

Физиология и биохимия растений

Plant Physiology and Biochemistry

The protective effects of Elettaria cardamomum essential oil extracted against Decadron induced on pancreatic tissues in rats (Rattus norvegicus)

Jabbar

Zahraa Abd Alreda

imankadhim28@gmail.com Al-Derawi

Karim H.

karim.thamir@uobasrah.edu.iq

0000-0002-1939-9838

Saadi

Sahar A. A. Malik Al

sahar.malik@uobasrah.edu.iq

0000-0003-2782-3907

College of Science, University of Basrah (Basrah, Iraq)

30 11 2025

2025

17 5 97 118 25 12 2024 16 03 2025 20 02 2025

2025

Z.A.A. Jabbar, K.H. Al-Derawi, S.A.A.M. Al Saadi

CC BY-NC-ND 4.0

https://discover-journal.ru/jour/index.php/sjlsa/article/view/1251

Background. Decadron is used to treat a wide range of disorders and regulates various physiological systems in the body. Literature data indicate that the essential oils of E. cardamomum exhibit multiple biological effects, including antihypertensive, antioxidant, antimicrobial properties, and pancreatic tissue protection. Purpose. This study aims to investigate the protective effects of E. cardamomum essential oils on body weight loss, hyperinsulinemia, hyperglycemia, and histopathological changes in the pancreas of rats induced by Decadron. Additionally, the chemical composition of the essential oils was characterized using GC-MS. Methodology. Rats were divided into six groups (n=5): (1) control, (2) Decadron-induced diabetes (1 mg/kg, orally), (3) induced diabetes + low-dose E. cardamomum essential oil (50 mg/kg, orally), (4) induced diabetes + high-dose E. cardamomum essential oil (100 mg/kg, orally), and (5 & 6) two groups receiving essential oils alone (50 & 100 mg/kg, orally). Diabetes was induced by administering Decadron (1 mg/kg, orally) for 14 days. Body weight was measured, and histopathological examination of pancreatic tissues was performed. Results. Decadron administration led to a decrease in body weight and an increase in glucose and insulin levels. Histopathological examination revealed hypertrophy of the islets of Langerhans, disintegration of pancreatic cells, and hemorrhage within pancreatic tissue. However, these pathological changes were mitigated by E. cardamomum essential oil treatment. GC-MS analysis identified 29 volatile compounds in the essential oils. Conclusion. Our findings suggest that cardamom oil may serve as an adjuvant in reducing elevated serum insulin and blood glucose levels in Decadron-induced diabetes.

Keywords Decadron Elletaria cardamomum hyperinsulinemia hyperglycemia GC-MS Eucalyptol pancreas

1. Suh, S., & Park, M. K. (2017). Glucocorticoid-induced diabetes mellitus: An important but overlooked problem. Endocrinology and Metabolism, 32(2), 180–189.

2. Hammadi Jasim, N., Adel Kareem, D., Majeed Al Ali, M. F., & Abbas, B. A. (2022). Effect of long-term treatment with dexamethasone on the liver and kidney histopathology, as well as blood biochemistry in male rabbits (Oryctolagus cuniculus). Archives of Razi Institute, 77(1), 333–343.

3. Haythorne, E., Rohm, M., van de Bunt, M., Brereton, M. F., Tarasov, A. I., Blacker, T. S., Sachse, G., Silva dos Santos, M., Terron Exposito, R., Davis, S., Baba, O., Fischer, R., Duchen, M. R., Rorsman, P., MacRae, J. I., & Ashcroft, F. M. (2019). Diabetes causes marked inhibition of mitochondrial metabolism in pancreatic β-cells. Nature Communications, 10(1), 2474–2491.

4. Kooptiwut, S., Samon, K., Semprasert, N., Suksri, K., & Yenchitsomanus, P.-T. (2020). Prunetin protects against dexamethasone-induced pancreatic β-cell apoptosis via modulation of p53 signaling pathway. Natural Product Communications, 15(4). https://doi.org/10.1177/1934578X20916328. EDN: https://elibrary.ru/JOTQKS

5. Li, J.-X., & Cummins, C. L. (2022). Fresh insights into glucocorticoid-induced diabetes mellitus and new therapeutic directions. Nature Reviews Endocrinology, 18(9), 540–557. https://doi.org/10.1038/s41574-022-00683-6. EDN: https://elibrary.ru/IMUKHX

6. Ali, R., Abbas, N., Abbas, A., & Abbas, L. (2020). Histological sections of pancreas and serum biochemical changes in rats after dexamethasone and zinc oxide nanoparticles injection. Medico-Legal Update, 20(1), 694–699.

7. Hamza, A. M., Hassan, M. S., Mousa, R. F., Jahil, A. H., & Hassan, M. S. (2022). Effect of aloe vera on antioxidant in male rats exposed to high doses of dexamethasone therapy, and histological on liver and pancreas. International Journal of Health Sciences, 6(6S), 8050–8062.

8. Glišić, R., Čakić-Milošević, M., Ukropina, M., Mitrovski-Bogdanović, A., & Stanković, V. (2023). Histological, ultrastructural and stereological analyses of pancreatic islets in glucocorticoid-treated rats. Kragujevac Journal of Science, 45, 179–194.

9. Oche, J., Olorundare, O., Afolabi, S., Ologe, M., Njan, A., & Akanbi, O. (2023). Comparative therapeutic effect of single/combined administration of saxagliptin, metformin and intranasal insulin on dexamethasone-induced insulin resistance in albino Wistar rat model. Nigerian Journal of Physiological Sciences, 38(1), 37–46.

10. Ashokkumar, K., Murugan, M., Dhanya, M. K., & Warkentin, T. D. (2020). Botany, traditional uses, phytochemistry and biological activities of cardamom [Elettaria cardamomum (L.) Maton] — a critical review. Journal of Ethnopharmacology, 246, 112244.

11. Dhungana, S. M., Regmi, P. P., Dhakal, S. C., et al. (2024). Growth instability index, and decomposition effect on production of large cardamom in Nepal. Agriculture & Food Security, 13, 5. https://doi.org/10.1186/s40066-023-00456-y. EDN: https://elibrary.ru/OWSGJJ

12. Shareef, A. (2018). Evaluation of antibacterial activity of essential oils of Cinnamomum sp. and Boswellia sp. Journal of Basrah Researches, 37(5), 60–71.

13. Husein, H. A., Alhasan, D. A. H., & Albadry, M. A. Z. (2019). In vitro antimicrobial activity of aqueous-methanolic extract of Populus sp. leaves. Basrah Journal of Agricultural Sciences, 31(2), 53–64.

14. Elmal, T. I. J., Mountassif, D., & Amarouch, H. (2007). Antimicrobial activity of Elettaria cardamomum: Toxicity, biochemical and histological studies. Food Chemistry, 104(4), 1560–1568.

15. Anwar, F., Abbas, A., Alkharfy, K. M., & Gilani, A.-H. (2016). Cardamom (Elettaria cardamomum Maton) oils. In Essential Oils in Food Preservation, Flavor and Safety (pp. 295–301).

16. Tarfaoui, K., Brhadda, N., Ziri, R., Oubihi, A., Imtara, H., Haida, S., Al Kamaly, O. M., Saleh, A., Parvez, M. K., Fettach, S., & Ouhssine, M. (2022). Chemical profile, antibacterial and antioxidant potential of Zingiber officinale Roscoe and Elettaria cardamomum (L.) Maton essential oils and extracts. Plants, 11(11), 1487–1501. https://doi.org/10.3390/plants11111487. EDN: https://elibrary.ru/OCIDBO

17. Attia, H., Alzoubi, A., Nour Al-Anazi, Alshanwani, A., El-Orabi, N., Alanteet, A., Mohamad, R., & Ali, R. (2023). Protective effects of cardamom aqueous extract against tamoxifen-induced pancreatic injury in female rats. Toxicological Research, 39(4), 721–737. https://doi.org/10.1007/s43188-023-00198-w. EDN: https://elibrary.ru/YIFCKR

18. Olivero-Verbel, J., González-Cervera, T., Güette-Fernandez, J., Jaramillo-Colorado, B., & Stashenko, E. (2010). Chemical composition and antioxidant activity of essential oils isolated from Colombian plants. Revista Brasileira de Farmacognosia, 20(4), 568–574.

19. Joshi, R., Sharma, P., Sharma, V., Prasad, R., Sud, R. K., & Gulati, A. (2012). Analysis of the essential oil of large cardamom (Amomum subulatum Roxb.) growing in different agro-climatic zones of Himachal Pradesh, India. Journal of the Science of Food and Agriculture, 93(6), 1303–1309.

20. Abdullah, Asghar, A., Butt, M. S., Shahid, M., & Huang, Q. (2017). Evaluating the antimicrobial potential of green cardamom essential oil focusing on quorum sensing inhibition of Chromobacterium violaceum. Journal of Food Science and Technology, 54(8), 2306–2315. https://doi.org/10.1007/s13197-017-2668-7. EDN: https://elibrary.ru/EJAVPJ

21. Jabbar, M., & Ghorbaniparvar, H. (2015). Use of GC-MS combined with resolution methods to characterize and to compare the essential oil components of green and bleached cardamom. Journal of Research in Chemistry and Environment, 5, 76–85.

22. Ghimire, B. K., Yoo, J. H., Yu, C. Y., & Chung, I. (2017). GC-MS analysis of volatile compounds of Perilla frutescens Britton var. japonica accessions: Morphological and seasonal variability. Asian Pacific Journal of Tropical Medicine, 10(7), 643–651.

23. Nurcholis, W., Sya’bani Putri, D. N., Husnawati, H., Aisyah, S. I., & Priosoeryanto, B. P. (2021). Total flavonoid content and antioxidant activity of ethanol and ethyl acetate extracts from accessions of Amomum compactum fruits. Annals of Agricultural Sciences, 66(1), 58–62. https://doi.org/10.1016/j.aoas.2021.04.001. EDN: https://elibrary.ru/MWJZSY

24. Zeedan, G., Abdalhamed, A., Ottai, M., Abdelshafy, S., & Abdeen, E. (2014). Antimicrobial, antiviral activity and GC-MS analysis of essential oil extracted from Achillea fragrantissima plant growing in Sinai Peninsula, Egypt. Journal of Microbial & Biochemical Technology, S8(01), 6–8.

25. Mottaghi, M., Parvin, S. S., Jafari, A. A., Mirza, M., & Bihamta, M. R. (2016). Essential oil composition of Achillea filipendulina, A. arabica and A. eriophora cultivated under temperate climate in Iran. Journal of Medicinal Plants and By-Products, 5(2), 153–158.

26. Toker, Z., Özen, H. Ç., Clery, R. A., & Owen, N. E. (2003). Essential oils of two Achillea species from Turkey. Journal of Essential Oil Research, 15(2), 100–110.

27. El Sayed Saleh, Shroq Al-Thbity, & Gobouri, A. (2022). Impact of extraction methods on the chemical composition and biological activity of small cardamom essential oils. Egyptian Journal of Chemistry, 65(2), 101–109.

28. Singh, G., Kiran, S., Marimuthu, P., Isidorov, V., & Vinogorova, V. (2007). Antioxidant and antimicrobial activities of essential oil and various oleoresins of Elettaria cardamomum (seeds and pods). Journal of the Science of Food and Agriculture, 88(2), 280–289.

29. Sultana, S., Ali, M., Ansari, S. H., & Bagri, P. (2009). Effect of physical factors on the volatile constituents of Elettaria cardamomum fruits. Journal of Essential Oil Bearing Plants, 12(3), 287–292.

30. Mehyar, G. F., Al-Isamil, K. M., Al-Ghizzawi, H. M., & Holley, R. A. (2014). Stability of cardamom (Elettaria cardamomum) essential oil in microcapsules made of whey protein isolate, guar gum, and carrageenan. Journal of Food Science, 79(10), C1939–C1949. https://doi.org/10.1111/1750-3841.12652. EDN: https://elibrary.ru/USZEJD

31. Menon, A. N., Chacko, S., & Narayanan, C. S. (1999). Free and glycosidically bound volatiles of cardamom (Elettaria cardamomum Maton var. miniscula Burkill). Flavour and Fragrance Journal, 14, 65–68.

32. Pavarino, M., Marengo, A., Cagliero, C., Bicchi, C., Rubiolo, P., & Sgorbini, B. (2023). Elettaria cardamomum (L.) Maton essential oil: An interesting source of bioactive specialized metabolites as inhibitors of acetylcholinesterase and butyrylcholinesterase. Plants, 12(19), 1–11.

33. Al-Zereini, W. A., Al-Trawneh, I. N., Al-Qudah, M. A., TumAllah, H. M., Al Rawashdeh, H. A., & Abudayeh, Z. H. (2022). Essential oils from Elettaria cardamomum (L.) Maton grains and Cinnamomum verum J. Presl barks: Chemical examination and bioactivity studies. Journal of Pharmacy & Pharmacognosy Research, 10(1), 173–185. https://doi.org/10.56499/jppres21.1162_10.1.173. EDN: https://elibrary.ru/YBNVOQ

34. Adams, R. (2017). Identification of essential oil components by gas chromatography/mass spectrometry (5th ed.). Allured Publishing.

35. Al-Saad, O. A., & Al-Saadi, M. (2021). Chemical composition and antioxidants of Lepidium sativum and L. aucheri. University of Thi-Qar Journal of Science, 8(1), 39–47.

36. Abd-Alrasoul, S. M., & Al-Saadi, M. (2022). Chemical composition and antioxidants of Artemisia herba-alba (Asteraceae). Iranian Journal of Ichthyology, 9(1), 302–308.

37. Skotti, E., Anastasaki, E., Kanellou, G., Polissiou, M., & Tarantilis, P. A. (2014). Total phenolic content, antioxidant activity and toxicity of aqueous extracts from selected Greek medicinal and aromatic plants. Industrial Crops and Products, 53, 46–54.

38. Bouaziz, A., Khennouf, S., Zarga, M. A., Abdalla, S., Baghiani, A., & Charef, N. (2015). Phytochemical analysis, hypotensive effect and antioxidant properties of Myrtus communis L. growing in Algeria. Asian Pacific Journal of Tropical Biomedicine, 5(1), 19–28.

39. Qader, K. O., Al-Saadi, S. A. A. M., & Faraj, I. M. (2018). Phytochemical constituents of leaves essential oils of Achillea fragrantissima (Asteraceae) from Iraq. ARO — The Scientific Journal of Koya University, 6(2), 56.

40. Al-Tamimi, W., Al-Saadi, S., & Burghal, A. (2020). Antibacterial activity and GC-MS analysis of Baltic amber against pathogenic bacteria. International Journal of Advanced Science and Technology, 29(11S), 611–618.

41. Farhat, G. N., Affara, N. I., & Gali-Muhtasib, H. U. (2001). Seasonal changes in the composition of the essential oil extract of East Mediterranean sage (Salvia libanotica) and its toxicity in mice. Toxicon, 39(10), 1601–1605. EDN: https://elibrary.ru/AXOEET

42. Kepta, F. A., Medou, F. M., Nyunaï, N. N., Kowa, T. K., Nguimmo-Metsadjio, A., & Tom, E. N. L. (2021). Antihyperglycemic, antioxidant, and organ protective effects of Schumanniophyton magnificum stem bark aqueous extract in dexamethasone-induced insulin resistance rats. GSC Advanced Research and Reviews, 9(3), 114–124. https://doi.org/10.30574/gscarr.2021.9.3.0295. EDN: https://elibrary.ru/NCFJMD

43. Ghadhban, R. F. (2024). Effect of dexamethasone, estrogen administration on leptin, thyroid, reproductive hormone concentration and lipid profile of female rabbits’ serum. Basrah Journal of Veterinary Research, 12(1), 41–53.

44. Hussei, A. J., Majeed, M. F., & Abbas, A. S. (2014). Histopathological study of some organ after long-term treatment with dexamethasone in male rabbits. Journal of University of Zakho, 2(1), 39–48.

45. Liu, C., Guan, J., Kang, Y., Xiu, H., Chen, Y., Deng, B., & Liu, K. (2010). Inhibition of dehydration-induced water intake by glucocorticoids is associated with activation of hypothalamic natriuretic peptide receptor-A in rat. PLOS ONE, 5(12), e15607.

46. Franco-Colín, M., Villanueva, I., Piñón, M., & Racotta, R. (2006). The effects of sympathectomy and dexamethasone in rats ingesting sucrose. International Journal of Biological Sciences, 6, 17–22.

47. Elamin, F., Abdel, A. K., & Dousa, M. (2017). Response of broiler chicks to dietary cardamom (Elettaria cardamomum) as a feed additive. Journal of Veterinary Medicine and Animal Production, 2(2), 1–10.

48. Shinde, S., Burte, R. G., Kumar, S., Desai, B., & Bhagat, D. (2017). Effect of cardamom (Elettaria cardamomum) and ginger (Zingiber officinale) powder supplementation on growth performance and economic analysis in broiler. International Journal of Chemical Studies, 5(3), 858–861.

49. Adanma, O., Okolie, N., & Michael, U. (2019). Histomorphological effects of Citrus aurantifolia (lime) leaf extract on acetaminophen-induced hepatotoxicity in Wistar rats. International Journal of Innovative Research and Advanced Studies, 6(6), 26–30.

50. Ashida, H., Furuyashiki, T., Nagayasu, H., Bessho, H., Sakakibara, H., Hashimoto, T., & Kanazawa, K. (2004). Anti-obesity actions of green tea: Possible involvements in modulation of the glucose uptake system and suppression of the adipogenesis-related transcription factors. BioFactors, 22(1–4), 135–140.

51. Abood, A. H., Al-Ali, I. A., & Hussein, K. A. (2022). Evaluation of the antidiabetic activity of a traditional herbal mixture in alloxan-induced diabetic rabbits. Journal of Hunan University Natural Sciences, 49(1), 149–157. https://doi.org/10.55463/issn.1674-2974.49.1.19. EDN: https://elibrary.ru/BMISCS

52. Sultana, A., Das, B. K., & Saha, D. (2024). Role of hentriacontane on dexamethasone-induced insulin resistance in rats. Pharmacological Research — Natural Products, 4, 100063. https://doi.org/10.1016/j.prenap.2024.100063. EDN: https://elibrary.ru/WRLIHX

53. Shittu, S.-T. T., Lasisi, T. J., Shittu, S. A.-S., Adeyemi, A., Adeoye, T. J., & Alada, A. A. (2021). Ocimum gratissimum enhances insulin sensitivity in male Wistar rats with dexamethasone-induced insulin resistance. Journal of Diabetes & Metabolic Disorders, 20(2), 1257–1267.

54. Holness, M. J., Smith, N. D., Greenwood, G. K., & Sugden, M. C. (2005). Interactive influences of peroxisome proliferator-activated receptor α activation and glucocorticoids on pancreatic beta cell compensation in insulin resistance induced by dietary saturated fat in the rat. Diabetologia, 48(10), 2062–2068. https://doi.org/10.1007/s00125-005-1894-0. EDN: https://elibrary.ru/DRNLWA

55. Rafacho, A., Roma, L. P., Taboga, S. R., Boschero, A. C., & Bosqueiro, J. R. (2007). Dexamethasone-induced insulin resistance is associated with increased connexin 36 mRNA and protein expression in pancreatic rat islets. Canadian Journal of Physiology and Pharmacology, 85(5), 536–545.

56. Kim, H. K., Park, H. R., Lee, J. S., Chung, T. S., Chung, H. Y., & Chung, J. (2007). Down-regulation of iNOS and TNF-α expression by kaempferol via NF-κB inactivation in aged rat gingival tissues. Biogerontology, 8(4), 399–408. https://doi.org/10.1007/s10522-007-9083-9. EDN: https://elibrary.ru/USVXVR

57. Yahyazadeh, R., Ghasemzadeh Rahbardar, M., Razavi, B. M., Karimi, G., & Hosseinzadeh, H. (2021). The effect of Elettaria cardamomum (cardamom) on the metabolic syndrome: Narrative review. Iranian Journal of Basic Medical Sciences, 24(11), 1462–1469.

58. Mahdavifard, S., & Nakhjavani, M. (2022). 1,8-cineole protects type 2 diabetic rats against diabetic nephropathy via inducing the activity of glyoxalase-I and lowering the level of transforming growth factor-1β. Journal of Diabetes & Metabolic Disorders, 21(1), 567–572. https://doi.org/10.1007/s40200-022-01014-2. EDN: https://elibrary.ru/WDIVXV

59. Rose, A., & Medou, F. (2020). Acute and subacute effects of aqueous extract of Picralima nitida seeds on dexamethasone-induced insulin resistance in rats. Annals of Biological Sciences, 8(1), 1–11.

60. Mahmoud, M. F., Elmaghraby, A. M., Ali, N., Mostafa, I., El-Shazly, A. M., Abdelfattah, M. A. O., & Sobeh, M. (2022). Black pepper oil (Piper nigrum L.) mitigates dexamethasone-induced pancreatic damage via modulation of oxidative and nitrosative stress. Biomedicine & Pharmacotherapy, 153, 113456.

61. Weir, G. C., Laybutt, D. R., Kaneto, H., Bonner-Weir, S., & Sharma, A. (2001). Beta-cell adaptation and decompensation during the progression of diabetes. Diabetes, 50(Suppl 1), S154–S159. https://doi.org/10.2337/diabetes.50.2007.s154

62. Barth, R., Ruoso, C., Ferreira, S. M. C., Lima, F. B., Boschero, A. C., & Jorge, G. (2021). Hepatocyte nuclear factor 4-α (HNF4α) controls the insulin resistance-induced pancreatic β-cell mass expansion. Life Sciences, 289, 120213.

63. Manlai, U., Chang, S.-W., Lee, S.-C., Ho, W.-J., Hsu, T.-H., Lin, J.-G., Lin, C.-M., Chen, Y.-I., & Chang, S.-L. (2021). Hypoglycemic effect of electroacupuncture combined with Antrodia cinnamomea in dexamethasone-induced insulin-resistant rats. Medical Acupuncture, 33(1), 58–64. https://doi.org/10.1089/acu.2020.1455. EDN: https://elibrary.ru/NVUKBN

64. Dwi, A., Jap, A., Lady, J., Prismawan, D., Sharopov, F., Daoud, R., Wink, M., & Sobeh, M. (2021). Function of selected natural antidiabetic compounds with potential against cancer via modulation of the PI3K/AKT/mTOR cascade. Biomedicine & Pharmacotherapy, 144, 112138.

65. Dawood, M., & Alkalby, J. (2020). Effect of treatment with dexamethasone on thyroid function in lactating female rats. Basrah Journal of Veterinary Research, 19(1), 331–345.

66. Amran, D., Al-Derawi, K., & Al-Saadi, S. (2023). Phytochemical constituents of bark essential oils of Cinnamomum zeylanicum Blume and effects on liver tissue of rats. University of Thi-Qar Journal of Science, 10(2), 165–170. https://doi.org/10.32792/utq/utjsci/v10i2.1131. EDN: https://elibrary.ru/QRLEHL

67. Majdalawieh, A. F., & Carr, R. I. (2010). In vitro investigation of the potential immunomodulatory and anti-cancer activities of black pepper (Piper nigrum) and cardamom (Elettaria cardamomum). Journal of Medicinal Food, 13(2), 371–381.

68. Rahman, M. M., Alam, M. N., Ulla, A., Sumi, F. A., Subhan, N., Khan, T., Sikder, B., Hossain, H., Reza, H. M., & Alam, M. A. (2017). Cardamom powder supplementation prevents obesity, improves glucose intolerance, inflammation and oxidative stress in liver of high carbohydrate high fat diet-induced obese rats. Lipids in Health and Disease, 16(1), 151. https://doi.org/10.1186/s12944-017-0539-x. EDN: https://elibrary.ru/YHBKTI

69. Aghasi, M., Koohdani, F., Qorbani, M., Nasli-Esfahani, E., Ghazi-Zahedi, S., Khoshamal, H., Keshavarz, A., & Sotoudeh, G. (2019). Beneficial effects of green cardamom on serum SIRT1, glycemic indices and triglyceride levels in patients with type 2 diabetes mellitus: A randomized double-blind placebo-controlled clinical trial. Journal of the Science of Food and Agriculture, 99(8), 3933–3940.

70. Daneshi-Maskooni, M., Keshavarz, S. A., Qorbani, M., Mansouri, S., Alavian, S. M., Badri-Fariman, M., Jazayeri-Tehrani, S. A., & Sotoudeh, G. (2019). Green cardamom supplementation improves serum irisin, glucose indices, and lipid profiles in overweight or obese non-alcoholic fatty liver disease patients: A double-blind randomized placebo-controlled clinical trial. BMC Complementary and Alternative Medicine, 19(1), 1–11. https://doi.org/10.1186/s12906-019-2465-0. EDN: https://elibrary.ru/COFNNH

71. Suneetha, W. J., & Krishnakantha, T. P. (2005). Cardamom extract as inhibitor of human platelet aggregation. Phytotherapy Research, 19(5), 437–440.

72. Goyal, S., Sharma, C., Mahajan, U., Patil, C., Agrawal, Y., Kumari, S., Arya, D., & Ojha, S. (2015). Protective effects of cardamom in isoproterenol-induced myocardial infarction in rats. International Journal of Molecular Sciences, 16(11), 27457–27469.

73. Kanthlal, S. K., Joseph, J., Paul, B. M., & P, U. D. (2020). Antioxidant and vasorelaxant effects of aqueous extract of large cardamom in L-NAME-induced hypertensive rats. Clinical and Experimental Hypertension, 42(7), 581–589. https://doi.org/10.1080/10641963.2020.1739699. EDN: https://elibrary.ru/AKWDYW

74. Issa, A. A., Alshami, J., & Al-Salih, H. A. H. (2023). Effect of dexamethasone on tail regeneration in the electric black ghost knifefish Apteronotus albifrons (Linnaeus, 1766). Basrah Journal of Agricultural Sciences, 36(2), 68–80. https://doi.org/10.37077/25200860.2023.36.2.06. EDN: https://elibrary.ru/OQKFKZ