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<article xmlns:mml="http://www.w3.org/1998/Math/MathML" xmlns:xlink="http://www.w3.org/1999/xlink" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:ali="http://www.niso.org/schemas/ali/1.0/" article-type="research-article" dtd-version="1.2" xml:lang="ru"><front><journal-meta><journal-id journal-id-type="publisher-id">Siberian Journal of Life Sciences and Agriculture</journal-id><journal-title-group><journal-title xml:lang="en">Siberian Journal of Life Sciences and Agriculture</journal-title><trans-title-group xml:lang="ru"><trans-title>Siberian Journal of Life Sciences and Agriculture</trans-title></trans-title-group></journal-title-group><issn publication-format="print">2658-6649</issn><issn publication-format="electronic">2658-6657</issn><publisher><publisher-name xml:lang="en">Science and Innovation Center Publishing House</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="publisher-id">442661</article-id><article-id pub-id-type="doi">10.12731/2658-6649-2026-18-1-1416</article-id><article-id pub-id-type="edn">GLQLNN</article-id><article-categories><subj-group subj-group-type="toc-heading" xml:lang="en"><subject>Plant Physiology and Biochemistry</subject></subj-group><subj-group subj-group-type="toc-heading" xml:lang="ru"><subject>Физиология и биохимия растений</subject></subj-group><subj-group subj-group-type="article-type"><subject>Research Article</subject></subj-group></article-categories><title-group><article-title xml:lang="en">Evaluation of morpho-physiological traits of Olea europaea L. associated with the drought-resistance</article-title><trans-title-group xml:lang="ru"><trans-title>Оценка морфо-физиологических признаков листьев Olea europaea L. в связи с засухоустойчивостью</trans-title></trans-title-group></title-group><contrib-group><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-1678-014X</contrib-id><contrib-id contrib-id-type="scopus">55799874500</contrib-id><contrib-id contrib-id-type="researcherid">HJZ-0652-2023</contrib-id><contrib-id contrib-id-type="spin">6975-4327</contrib-id><name-alternatives><name xml:lang="ru"><surname>Цюпка</surname><given-names>Сергей Юрьевич</given-names></name><name xml:lang="en"><surname>Tsiupka</surname><given-names>Sergei Y.</given-names></name></name-alternatives><address><country country="RU">Россия</country></address><bio xml:lang="ru"><p>кандидат сельскохозяйственных наук, старший научный сотрудник отдела плодовых культур</p> <p> </p></bio><bio xml:lang="en"><p>PhD, Senior Researcher</p> <p> </p></bio><email>tsupkanbg@mail.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0002-9929-0946</contrib-id><contrib-id contrib-id-type="scopus">56811269600</contrib-id><contrib-id contrib-id-type="researcherid">ABA-2273-2020</contrib-id><contrib-id contrib-id-type="spin">2834-9125</contrib-id><name-alternatives><name xml:lang="ru"><surname>Булавин</surname><given-names>Илья Владимирович</given-names></name><name xml:lang="en"><surname>Bulavin</surname><given-names>Iliya V.</given-names></name></name-alternatives><address><country country="RU">Россия</country></address><bio xml:lang="ru"><p>кандидат биологических наук, заведующий лабораторией клеточной биологии и анатомии, старший научный сотрудник</p> <p> </p></bio><bio xml:lang="en"><p>PhD, Head of the Laboratory of Plant Cell Biology and Anatomy, Senior Researcher</p> <p> </p></bio><email>cellbiolnbs@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0009-0000-4079-2418</contrib-id><name-alternatives><name xml:lang="ru"><surname>Синченко</surname><given-names>Анастасия Вячеславовна</given-names></name><name xml:lang="en"><surname>Sinchenko</surname><given-names>Anastasia V.</given-names></name></name-alternatives><address><country country="RU">Россия</country></address><bio xml:lang="ru"><p>младший научный сотрудник лаборатории Курчатовский геномный центр – НБС-ННЦ</p> <p> </p></bio><bio xml:lang="en"><p>Junior Researcher, Laboratory of Plant Genomics and Bioinformatics</p> <p> </p></bio><email>nas.sin4enko@gmail.com</email><xref ref-type="aff" rid="aff1"/></contrib><contrib contrib-type="author"><contrib-id contrib-id-type="orcid">https://orcid.org/0000-0003-3853-0210</contrib-id><contrib-id contrib-id-type="scopus">57190663916</contrib-id><contrib-id contrib-id-type="researcherid">AAS-6393-2020</contrib-id><contrib-id contrib-id-type="spin">2173-9806</contrib-id><name-alternatives><name xml:lang="en"><surname>Tsiupka</surname><given-names>Valentina A.</given-names></name><name xml:lang="ru"><surname>Цюпка</surname><given-names>Валентина Анатольевна</given-names></name></name-alternatives><address><country country="RU">Россия</country></address><bio xml:lang="ru"><p>кандидат биологических наук, ведущий научный сотрудник лаборатории Курчатовский геномный центр – НБС-ННЦ</p> <p> </p></bio><bio xml:lang="en"><p>PhD, Leading Researcher of the Laboratory of the Kurchatov Genome Center – NBG-NSC</p> <p> </p></bio><email>valentina.brailko@yandex.ru</email><xref ref-type="aff" rid="aff1"/></contrib></contrib-group><aff-alternatives id="aff1"><aff><institution xml:lang="ru">ФГБУН «Никитский ботанический сад-Национальный научный центр»</institution></aff><aff><institution xml:lang="en">Nikitsky Botanical Garden-National Scientific Center</institution></aff></aff-alternatives><content-language>ru</content-language><pub-date date-type="pub" iso-8601-date="2026-02-28" publication-format="electronic"><day>28</day><month>02</month><year>2026</year></pub-date><pub-date date-type="collection"><year>2026</year></pub-date><volume>18</volume><issue>1</issue><issue-title xml:lang="ru"/><fpage>83</fpage><lpage>114</lpage><history><date date-type="received" iso-8601-date="2026-07-30"><day>30</day><month>07</month><year>2026</year></date></history><permissions><copyright-statement xml:lang="en">Copyright ©; 2026, Tsiupka S.Y., Bulavin I.V., Sinchenko A.V., Tsiupka V.A.</copyright-statement><copyright-statement xml:lang="ru">Copyright ©; 2026, Цюпка С.Ю., Булавин И.В., Синченко А.В., Цюпка В.А.</copyright-statement><copyright-year>2026</copyright-year><copyright-holder xml:lang="en">Tsiupka S.Y., Bulavin I.V., Sinchenko A.V., Tsiupka V.A.</copyright-holder><copyright-holder xml:lang="ru">Цюпка С.Ю., Булавин И.В., Синченко А.В., Цюпка В.А.</copyright-holder><ali:free_to_read xmlns:ali="http://www.niso.org/schemas/ali/1.0/"/><license><ali:license_ref xmlns:ali="http://www.niso.org/schemas/ali/1.0/">https://creativecommons.org/licenses/by-nc-nd/4.0</ali:license_ref></license></permissions><self-uri xlink:href="https://journals.rcsi.science/2658-6649/article/view/442661">https://journals.rcsi.science/2658-6649/article/view/442661</self-uri><abstract xml:lang="en"><p>Background. Olive (Olea europaea L.) is one of the main fruit crops in the world. According to IOC (International olive council) the world olive cultivation is estimated at 10.9 million hectares, most of which is located in the zone of insufficient water supply. The development of new high-yielding varieties tolerant to drought is one of the most effective strategies to increase olive yields. However, breeding for drought tolerance is complicated by the lack of rapid, reproducible screening methods due to the involvement of a number of physiological and morphological traits in the response.</p> <p>Purpose. Determine the morphological and physiological characteristics associated with drought resistance of olives and suitable for mass analysis of breeding material.</p> <p>Materials and methods. The experiments were performed in 2022-2024 on 60-year-old trees planted 5x5 m apart, with a planting density of 400 trees per ha and southern exposure of the slope. 14 olive genotypes different by origins were selected. Structural and physiological investigation, as well as statistical analysis, was conducted according to the generally accepted method.</p> <p>Results. Leaf morphological traits were studied to reduce water loss in 14 European olive genotypes. Leaf measurements included the following leaf characteristics: density of stomata and trichomes, leaf length, width and area, leaf mass per unit area, leaf tissue density, etc., as well as physiological parameters: leaf water-holding capacity, cuticular transpiration rate and relative electrolyte yield observed during cell membrane damage. The highest correlation coefficients for the relative electrolyte yield were noted with the stomatal slit area (r=0.89) and the trichome area index (r=-0.85). According to the Chaddock correlation scale, such coefficients indicate a high dependence between these parameters.</p></abstract><trans-abstract xml:lang="ru"><p>Обоснование. Маслина европейская (Olea europaea L.) является одной из основных плодовых культур в мире. Мировое выращивание оливок оценивается в 10,9 млн гектаров, большая часть из которых находится в зоне недостаточного водообеспечения. Разработка новых высокоурожайных сортов, устойчивых к засухе, является одной из наиболее эффективных стратегий повышения урожайности маслины. Однако селекция по данному признаку осложняется отсутствием быстрых, воспроизводимых методов скрининга устойчивости к гидротермическому стрессу из-за вовлечения в реакцию ряда физиологических и морфологических признаков.</p> <p>Цель – определить морфологические и физиологические признаки, связанные с засухоустойчивостью маслины европейской и пригодные для массового анализа селекционного материала.</p> <p>Материалы и методы. Исследования проводили в 2022-2024 гг. на 60-летних деревьях, с плотностью посадки 400 деревьев на га и южной экспозицией склона. В качестве объектов были выбраны 14 генотипов O. europaea различного генетического происхождения. Структурные и физиологические исследования, а также статистический анализ выполняли общепринятым способом.</p> <p>Результаты. Выявлена зависимость между морфологическими характеристиками листа, показателями водного режима растений и относительным выходом электролита. Самые высокие коэффициенты корреляции отмечены <ext-link/>между относительным выходом электролита и площадью устьичной щели (r=0,89), а также индексом площади трихом (r=-0,85). По шкале корреляции Чеддока такие коэффициенты говорят о высокой зависимости между данными параметрами.</p> <p>Заключение. Отмечена значительная вариация морфологических признаков, ассоциированных с засухоустойчивостью, некоторые из которых могут быть использованы в качестве биомаркеров для дифференциации сортов и гибридов маслины. Полученные результаты исследований могут быть использованы при скрининге селекционных коллекций O. europaea на признак засухоустойчивости.</p></trans-abstract><kwd-group xml:lang="ru"><kwd>маслина</kwd><kwd>стресс</kwd><kwd>дегидратация</kwd><kwd>засуха</kwd><kwd>устьица</kwd><kwd>трихомы</kwd><kwd>площадь листа</kwd></kwd-group><kwd-group xml:lang="en"><kwd>olive</kwd><kwd>stress</kwd><kwd>dehydration</kwd><kwd>drought</kwd><kwd>stomata</kwd><kwd>trichomes</kwd><kwd>leaf area</kwd></kwd-group><funding-group/></article-meta></front><body></body><back><ref-list><ref id="B1"><label>1.</label><citation-alternatives><mixed-citation xml:lang="en">Babosha, A. V., Ryabchenko, A. S., &amp; Kumakhova, T. Kh. (2023). 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