Multiantagonistic of Streptomyces narbonensis strain PSM242 and Trichoderma sp. biocide combined in enrich media against Meloidogyne sp. root‑knot nematode on cherry tomato plants
Аннотация
The research focuses on the isolation and identification of Streptomyces and Meloidogyne species from soil and plant samples, particularly in oil palm plantations and tomato crops. Using the soil plating method, two Streptomyces isolates were obtained, identified as Streptomyces narbonensis through molecular techniques targeting the 16S rRNA gene. Concurrently, root-knot nematodes (Meloidogyne sp.) were extracted from symptomatic tomato plants using a modified Baerman apparatus, with morphological characteristics confirming their identity. The study further evaluates the biocontrol potential of S. narbonensis strain PSM242 and Trichoderma sp. against Meloidogyne sp. through both in vitro and field experiments. Results indicate that these biocontrol agents significantly reduce nematode populations and root gall formation, leading to enhanced growth metrics in cherry tomato plants. A factorial randomized block design was employed for data analysis, revealing that the combination of S. narbonensis and Trichoderma sp. in nutrient-enriched media yielded optimal results in controlling nematodes. Statistical analyses demonstrated that treatments combining both biocontrol agents resulted in the lowest gall diameter, weight, and juvenile nematode populations compared to controls. Additionally, significant improvements in root length and fruit weight were observed in treated plants. This research underscores the potential of utilizing microbial antagonists as sustainable alternatives for managing agricultural pests, contributing to more effective pest control strategies in crop production systems.
EDN: TQLSFD
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Литература
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