Isolation and identification of oil-degrading bacteria from oil-contaminated muddy soil samples at automotive service stations in Vietnam
Аннотация
Background. Oil pollution from vehicle maintenance and oil storage tanks at automotive service stations poses significant environmental challenges, affecting both soil and water ecosystems. Bioremediation is an effective and eco-friendly approach that utilizes microorganisms to degrade hydrocarbons in contaminated environments. Numerous indigenous bacterial species capable of hydrocarbon degradation have been studied for their potential application in pollution treatment.
Purpose. This study aimed to isolate and identify oil-degrading bacterial strains from oil-contaminated muddy soil samples collected from automotive service stations in Hanoi and Dong Nai, Vietnam. The objective was to evaluate their degradation efficiency and explore their potential application in bioremediation strategies.
Materials and methods. Four oil-contaminated muddy soil samples were collected from car wash areas and oil storage tanks in Hanoi and Dong Nai, Vietnam, in August 2024. In this study, we used methods such as: enrichment in GOST mineral medium supplemented with crude oil mixed in DO, isolation method, assessment of oil degradation ability by gravimetric methods, OD600nm measurement by UV-vis spectrophotometer, study of morphological characteristics and molecular identification of bacterial strains.
Results. From four oil-contaminated mud samples, after three enrichment cycles in a mineral medium supplemented with 5% (w/v) crude oil and diesel, sample M4 exhibited the highest oil degradation efficiency, achieving 80.12% removal after three enrichment cycles. Six representative bacterial strains were isolated on MPA agar from sample M4 and identified based on morphological and biochemical characteristics. Using molecular biological techniques, these hydrocarbon-degrading strains were identified as Achromobacter xylosoxidans ZB1.3 (PQ351236), Ignatzschineria rhizosphaerae ZB2.4 (PQ351237), Stenotrophomonas acidaminiphila ZB2.1 (PQ351238), Brevundimonas diminuta KN2.3 (PQ351239), Aeromonas hydrophila KN3.2 (PQ351240), and Rhodococcus ruber JN5.2 (PQ351241). The isolates, particularly strain JN5.2, demonstrated the ability to grow in a mineral medium supplemented with 1% oil after six days of incubation.
Conclusion. These results reveal the diversity of oil-degrading microorganisms and underscore the potential of indigenous microbial communities for self-remediation in oil-polluted environments, offering a sustainable and effective solution for environmental restoration.
EDN: KWGBLV
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Copyright (c) 2025 Thi Kim Thanh Nguyen, Thi Tuyen Do, Thi Thanh Thuy Tran, Thi Mo Luong, Quang Tuyen Mai, The Can Nguyen, Khac Trinh Nguyen, Dinh Dai Phan, Cao Cuong Ngo
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