POSSIBILITIES OF ISOLATION AND CULTIVATION OF GASTRIC EPITHELIAL CELLS
Abstract
Cultivation of cells of the gastric mucosa is quite difficult. However, the possibility of using epithelial cells as models for in vivo studies is quite promising.
The purpose. To obtain a primary culture of gastric mucosa cells and evaluate its phenotypic and metabolic characteristics.
Materials and methods. This paper presents the results of a methodological study on obtaining primary cell cultures of the human gastric mucosa by step-by-step proteolysis and the possibility of their further cultivation. Multicolour flow cytometry was used to evaluate the phenotype of the obtained cells and their viability.
Research result. We were isolated heterogeneous cells of the gastric mucosa with a high percentage of viability. During cultivation, the number of viable cells decreased slightly.
Conclusion. We obtained a primary viable culture of gastric epithelial cells, which allows us to conclude that the presented method of isolation is suitable for obtaining viable cells.
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References
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Copyright (c) 2021 Olga V. Peretyat'ko, Vasiliy D. Belenyuk, Olga L. Moskalenko
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